We are not currently accepting samples until the conclusion of our research study.

For a provider information packet, please email ccr5@ifi-test.com

Download our CCR5 Genetic Assay Brochure in Acrobat Reader.


TESTING DETAILS

METHOD

RESULTS

REFERENCES

ADDITIONAL RESOURCES

CCR5 GENETIC Assay FROM IFI  

The CCR5 Genetic Assay,
from Independent Forensics identifies normal genetic differences in the CCR5 gene.

These inherited genetic differences, define an individual's CCR5 genetics (called a haplotype by geneticists).

Scientific evidence demonstrating correlations between CCR5 genetics and HIV-1 disease progression probability rates has been established by a number of large, research studies funded by the
National Institute of Health
(see references below)

The CCR5 Genetic Assay from Independent Forensics identifies CCR5 genetic variations and correlates the determined CCR5 haplotype with a specific HIV-1 disease progression rate (slow, average, or fast).


CCR5 GENETIC TESTING DETAILS
Results processed in 3 weeks.

We are not currently accepting samples until the conclusion of our research study.

  • The results of the CCR5 Genetic Test should be discussed with a medical provider(s) as part of a complete plan for medical treatment, monitoring and health.

  • The correlation of the CCR5 Genetic Test results with associated HIV-1 disease progression are only valid for HIV-1 positive individuals.

  • Your CCR5 haplotype may not correlate with a known HIV-1 disease progression group.

  • The CCR5 Genetic Test will not determine HIV status.

  • The CCR5 Genetic Test will not determine CD-4 count.

  • The CCR5 Genetic Test will not determine viral load.

  • The CCR5 Genetic Test will not determine viral genotype or viral phenotype.
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CCR5 GENETIC TEST METHOD
  • Sample Collection
    Buccal (inner-cheek cell) swab collection on a sterile cotton tipped applicator (kit and instructions will be supplied)

  • DNA Extraction
    Single Tube Extraction method followed by amplification of DNA.

  • DNA Analysis
    Beckman Coulter CEQ8000 Genetic Analyzer is used to detect CCR5 Genetic Variants.  Nuclear DNA is tested for specific, defined, normal variations in the gene that codes for the HIV-1 coreceptor, CCR5.

  • CCR5 Genetic Test Validation Data: Available Soon.
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CCR5 GENETIC TEST RESULTS
This test will identify medically significant CCR5 alleles and haplotypes:

    Based on the normal variations in the CCR5 gene, eight CCR5 haplotype groups have been shown to be medically relevant:

    CCR5 Human Haplotypes:
    HHA, HHC, HHD, HHE, HHF*1, HHF*2, HHG*1, HHG*2(Delta-32)

The associated HIV-1 disease progression rate will be categorized into epidemiological classes based on the identified CCR5 haplotype.
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CCR5 GENETIC TEST REFERENCES

Gonzalez E, Bamshad M, Sato N et al.  1999. Race-specific HIV-1 disease modifying effects associated with CCR5 haplotypes. Proc. Natl. Acad. Sci. USA 96:12004-12009

Tang J, Shelton B, Makhatadze NJ, Zhang Y, Schaen M, Louie LG, Goedert JJ, Seaberg EC, Margolick JB, Mellors J, Kaslow RA. 2002. Distribution of Chemokine Receptor CCR2 and CCR5 Genotypes and Their Relative Contribution to Human Immunodeficiency Virus Type 1 (HIV-1) Seroconversion, Early HIV-1 RNA Concentration in Plasma, and Later Disease Progression. J.Virol. 76(2):662-672.

Dean M, Carrington M, Winkler C et al. 1996. Genetic restriction of HIV-1 infection and progression to AIDS by a common deletion allele of the chemokine receptor 5 structural gene.  Science 273:1856-1862.

Martin MP, Dean M, Smith MW, Winkler C et al. 1998.  Genetic acceleration of AIDS progression by a promoter variant of CCR5. Science 282:1907-1911.

Tang J, Rivers C, Karita E, Costello C, Allen S, Fultz PN, Schoenbaum EE, and Kaslow RA. 1999. Allelic variants of human beta-chemokine receptor 5 (CCR5) promoter: evolutionary relationships and predictable association with HIV-1 disease progression. Genes Immun. 1:20-27.

Mummidi S, Ahuja SS, Gonzalez E et al. 1998. Genealogy of the CCR5 locus and chemokine system gene variants associated with altered rates of HIV-1 disease progression.  Nat. Med. 4:786-793.
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ADDITIONAL RESOURCES
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