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SPERM HY-LITER Documentation



Sperm Hy-Liter™: an effective tool for the detection of spermatozoa in sexual assault exhibits. De Moors A, Georgalis T, Armstrong G, Modler J, Frégeau CJ. Forensic Sci Int Genet. 2013 May;7(3):367-79.

Low copy number DNA profiling from isolated sperm using the aureka®-micromanipulation system. Schneider C, Müller U, Kilper R, Siebertz B. Forensic Sci Int Genet. 2012 Jul;6(4):461-5.

Developmental validation of the SPERM HY-LITER™ kit for the identification of human spermatozoa in forensic samples. Miller KW, Old J, Fischer BR, Schweers B, Stipinaite S, Reich K. J Forensic Sci. 2011 Jul;56(4):853-65

Automatic detection of spermatozoa for laser capture microdissection. Vandewoestyne M, Van Hoofstat D, Van Nieuwerburgh F, Deforce D. Int J Legal Med. 2009 Mar;123(2):169-75.

Immunohistochemical staining of human sperm cells in smears from sexual assault cases. Christoffersen S. Int J Legal Med. 2011 Nov;125(6):887-90.


Download Validation Study revised 07/2010
Download Technical Information revised 07/2010
Download Protocol  revised 07/2010
Online Presentation
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  • Positive Identification of Sperm
  • Microscopic Screening of Sperm
  • Human Specific – Monoclonal Antibody-based
  • Fluorescent Detection – Debris Does Not Interfere
  • Meets New Audit Standards for Evidence Preservation
  • They Glow - Scan at 40X


Complete Fluorescent Staining Kit for Sexual Assault Evidence



Step #1: Attach Cells to Slide: 10 min incubation
Step #2: Sample Preparation: 30 min incubation
Step #3: Blocking: 30 min incubation
Step #4: Labeling & Staining: 30 min incubation
Step #5: Mounting & Cover Slip: 2 minutes


Hands-on Time: less than 5 minutes!

  • Protocols - Instructions - Technical Documentation - Included in Every Kit

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